Genotyping and primer details


Genotyping and primer details for SALK_061534c - At2g45920
Line ID SALK_061534c
Locus At2g45920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18900429 of line SALK_061534c Chr2:18900429
   
Insertion allele amplicon LBb3 - 62fr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 62fr: 5'-GGTCTACCCCTAGTCCCTGTAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18899988
   
wt amplicon (over InsPos) 01hs - 62fr
wt amplicon start Chr2:18899988
wt amplicon end Chr2:18900911
wt amplicon size 924 bp
Locus specific primer 62fr: 5'-GGTCTACCCCTAGTCCCTGTAATC-3'
2nd locus specific primer 01hs: 5'-CTTCATCACTTCCTGAAACATTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11