Genotyping and primer details


Genotyping and primer details for SALK_061645c - At1g23460
Line ID SALK_061645c
Locus At1g23460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8328413 of line SALK_061645c Chr1:8328413
   
Insertion allele amplicon LBb3 - 26di
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 26di: 5'-TTGATGATGTACCTGATTCCATGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8328849
   
wt amplicon (over InsPos) 12mu - 26di
wt amplicon start Chr1:8327865
wt amplicon end Chr1:8328849
wt amplicon size 985 bp
Locus specific primer 26di: 5'-TTGATGATGTACCTGATTCCATGT-3'
2nd locus specific primer 12mu: 5'-AAAGCCTGCAGTACATCAAAATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11