Genotyping and primer details


Genotyping and primer details for SALK_063269c - At3g57540
Line ID SALK_063269c
Locus At3g57540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21301629 of line SALK_063269c Chr3:21301629
   
Insertion allele amplicon LBb3 - 11ii
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 11ii: 5'-GTTTAAGAGACAAGACGCCGTTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21302316
   
wt amplicon (over InsPos) 09ir - 11ii
wt amplicon start Chr3:21301155
wt amplicon end Chr3:21302316
wt amplicon size 1162 bp
Locus specific primer 11ii: 5'-GTTTAAGAGACAAGACGCCGTTAT-3'
2nd locus specific primer 09ir: 5'-GTCTGAGCAAGATTTTAGTGGGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11