Genotyping and primer details


Genotyping and primer details for SALK_063674 - At1g15870
Line ID SALK_063674
Locus At1g15870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5457149 of line SALK_063674 Chr1:5457149
   
Insertion allele amplicon LBb3 - 17rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17rb: 5'-CATCAATTTGCGTATGAGATGATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5457511
   
wt amplicon (over InsPos) 16rz - 17rb
wt amplicon start Chr1:5456701
wt amplicon end Chr1:5457511
wt amplicon size 811 bp
Locus specific primer 17rb: 5'-CATCAATTTGCGTATGAGATGATT-3'
2nd locus specific primer 16rz: 5'-TTGACAATTTTGTTGAGTGGATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11