Genotyping and primer details


Genotyping and primer details for SALK_066378c - At2g33480
Line ID SALK_066378c
Locus At2g33480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14181357 of line SALK_066378c Chr2:14181357
   
Insertion allele amplicon LBb3 - 22md
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 22md: 5'-CAAGAACACTCTACACAAAACCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14181917
   
wt amplicon (over InsPos) 10nl - 22md
wt amplicon start Chr2:14180883
wt amplicon end Chr2:14181917
wt amplicon size 1035 bp
Locus specific primer 22md: 5'-CAAGAACACTCTACACAAAACCCA-3'
2nd locus specific primer 10nl: 5'-ATTTCGCTGTAACTTCGGTAAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11