Genotyping and primer details


Genotyping and primer details for SALK_067559c - At4g25620
Line ID SALK_067559c
Locus At4g25620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13067535 of line SALK_067559c Chr4:13067535
   
Insertion allele amplicon LBb3 - 17ii
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17ii: 5'-CTGGTTCAGGTACATCTTCTCCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13068150
   
wt amplicon (over InsPos) 05ir - 17ii
wt amplicon start Chr4:13067114
wt amplicon end Chr4:13068150
wt amplicon size 1037 bp
Locus specific primer 17ii: 5'-CTGGTTCAGGTACATCTTCTCCTT-3'
2nd locus specific primer 05ir: 5'-TCAGATCTTTTGTGCAATGAATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11