Genotyping and primer details


Genotyping and primer details for SALK_069427c - At3g04090
Line ID SALK_069427c
Locus At3g04090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1072800 of line SALK_069427c Chr3:1072800
   
Insertion allele amplicon LBb3 - 23md
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23md: 5'-TAGCCTTTTCTAGAGTTTCTTGCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1073235
   
wt amplicon (over InsPos) 35rd - 23md
wt amplicon start Chr3:1072320
wt amplicon end Chr3:1073235
wt amplicon size 916 bp
Locus specific primer 23md: 5'-TAGCCTTTTCTAGAGTTTCTTGCG-3'
2nd locus specific primer 35rd: 5'-TCCCAATTGTTTTATTTTCCTCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11