Genotyping and primer details


Genotyping and primer details for SALK_071365 - At3g59540
Line ID SALK_071365
Locus At3g59540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21996305 of line SALK_071365 Chr3:21996305
   
Insertion allele amplicon LBb3 - 14jd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 14jd: 5'-TAACGACTGCTTTTTCTCCAAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21995520
   
wt amplicon (over InsPos) 07km - 14jd
wt amplicon start Chr3:21995520
wt amplicon end Chr3:21996604
wt amplicon size 1085 bp
Locus specific primer 14jd: 5'-TAACGACTGCTTTTTCTCCAAAAT-3'
2nd locus specific primer 07km: 5'-CTTATTGTGGGTTTCTTGTTTGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11