Genotyping and primer details


Genotyping and primer details for SALK_071403c - At3g18035
Line ID SALK_071403c
Locus At3g18035 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6170385 of line SALK_071403c Chr3:6170385
   
Insertion allele amplicon LBb3 - 77xo
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 77xo: 5'-TCTCCATTCAAGTAACAACGATCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6170996
   
Insertion allele amplicon R204 - BI02
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BI02: 5'-CACTTGTAACTCCATCCTTCAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6169617
   
wt amplicon (over InsPos) 08cj - BI02
wt amplicon start Chr3:6169617
wt amplicon end Chr3:6170627
wt amplicon size 1011 bp
Locus specific primer BI02: 5'-CACTTGTAACTCCATCCTTCAACA-3'
2nd locus specific primer 08cj: 5'-CGTTTCTTCTACTGCCTCTGTGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11