Genotyping and primer details


Genotyping and primer details for SALK_071663c - At1g75400
Line ID SALK_071663c
Locus At1g75400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28298628 of line SALK_071663c Chr1:28298628
   
Insertion allele amplicon LBb3 - 50cy
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 50cy: 5'-TGAAAGACAGAAAGGTTCCTCCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28297634
   
wt amplicon (over InsPos) 13dz - 50cy
wt amplicon start Chr1:28297634
wt amplicon end Chr1:28298814
wt amplicon size 1181 bp
Locus specific primer 50cy: 5'-TGAAAGACAGAAAGGTTCCTCCTA-3'
2nd locus specific primer 13dz: 5'-ATCTGCTGCTACCACCACTTAGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11