Genotyping and primer details


Genotyping and primer details for SALK_073421 - At2g22450
Line ID SALK_073421
Locus At2g22450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9531159 of line SALK_073421 Chr2:9531159
   
Insertion allele amplicon R204 - BI68
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BI68: 5'-TCAAAGGAAAAATATGACCAGGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9531717
   
Insertion allele amplicon LBb3 - BI69
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer BI69: 5'-CTCGTCGTCTCTCTCTTCAAAACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9530621
   
wt amplicon (over InsPos) BI69 - BI68
wt amplicon start Chr2:9530621
wt amplicon end Chr2:9531717
wt amplicon size 1097 bp
Locus specific primer BI68: 5'-TCAAAGGAAAAATATGACCAGGAC-3'
2nd locus specific primer BI69: 5'-CTCGTCGTCTCTCTCTTCAAAACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11