Genotyping and primer details


Genotyping and primer details for SALK_074937 - At3g53930
Line ID SALK_074937
Locus At3g53930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19966782 of line SALK_074937 Chr3:19966782
   
Insertion allele amplicon LBb3 - 09bh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 09bh: 5'-TGTAACGGACACCAATAATTTCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19965967
   
wt amplicon (over InsPos) 36lt - 09bh
wt amplicon start Chr3:19965967
wt amplicon end Chr3:19967028
wt amplicon size 1062 bp
Locus specific primer 09bh: 5'-TGTAACGGACACCAATAATTTCAA-3'
2nd locus specific primer 36lt: 5'-AAGCTGACACATTTTTAGAATCCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11