Genotyping and primer details


Genotyping and primer details for SALK_074964c - At3g13070
Line ID SALK_074964c
Locus At3g13070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4194832 of line SALK_074964c Chr3:4194832
   
Insertion allele amplicon LBb3 - 60cs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 60cs: 5'-TTTAGCGAAGTTTCTAGCTCCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4195180
   
wt amplicon (over InsPos) 16dz - 60cs
wt amplicon start Chr3:4194339
wt amplicon end Chr3:4195180
wt amplicon size 842 bp
Locus specific primer 60cs: 5'-TTTAGCGAAGTTTCTAGCTCCAAG-3'
2nd locus specific primer 16dz: 5'-GATAAGCCGTACATAACACAGCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11