Genotyping and primer details


Genotyping and primer details for SALK_079505c - At5g29000
Line ID SALK_079505c
Locus At5g29000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:11023253 of line SALK_079505c Chr5:11023253
   
Insertion allele amplicon LBb3 - 13kw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 13kw: 5'-AAATTGGTCTGAACTTCTTGGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:11023711
   
wt amplicon (over InsPos) 17mu - 13kw
wt amplicon start Chr5:11022917
wt amplicon end Chr5:11023711
wt amplicon size 795 bp
Locus specific primer 13kw: 5'-AAATTGGTCTGAACTTCTTGGAGA-3'
2nd locus specific primer 17mu: 5'-AGGTTCTCCTGTGCAAGAAATAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11