Genotyping and primer details


Genotyping and primer details for SALK_079981c - At3g45310
Line ID SALK_079981c
Locus At3g45310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:16628738 of line SALK_079981c Chr3:16628738
   
Insertion allele amplicon LBb3 - 07jd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 07jd: 5'-TACATGTGGTAACACTCCAATGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:16629112
   
wt amplicon (over InsPos) 01kp - 07jd
wt amplicon start Chr3:16628367
wt amplicon end Chr3:16629112
wt amplicon size 746 bp
Locus specific primer 07jd: 5'-TACATGTGGTAACACTCCAATGGT-3'
2nd locus specific primer 01kp: 5'-AAAAATTACTCCCAAGTCCCAAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11