Genotyping and primer details


Genotyping and primer details for SALK_081127c - At5g44720
Line ID SALK_081127c
Locus At5g44720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18043352 of line SALK_081127c Chr5:18043352
   
Insertion allele amplicon LBb3 - 37cv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 37cv: 5'-ATCAAACAAGACCCTTTTCTGCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18043744
   
wt amplicon (over InsPos) 12go - 37cv
wt amplicon start Chr5:18042759
wt amplicon end Chr5:18043744
wt amplicon size 986 bp
Locus specific primer 37cv: 5'-ATCAAACAAGACCCTTTTCTGCTA-3'
2nd locus specific primer 12go: 5'-GGTGGTAGGTCGACCATTCTAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11