Genotyping and primer details


Genotyping and primer details for SALK_083158c - At4g33370
Line ID SALK_083158c
Locus At4g33370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16069687 of line SALK_083158c Chr4:16069687
   
Insertion allele amplicon LBb3 - 64us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 64us: 5'-AGTAACCAGTAGAGCCGGTGACTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16069305
   
wt amplicon (over InsPos) 59vl - 64us
wt amplicon start Chr4:16069305
wt amplicon end Chr4:16070296
wt amplicon size 992 bp
Locus specific primer 64us: 5'-AGTAACCAGTAGAGCCGGTGACTA-3'
2nd locus specific primer 59vl: 5'-AGCAAAGATTGTTTACCTTCTGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11