Genotyping and primer details


Genotyping and primer details for SALK_086372 - At1g23760
Line ID SALK_086372
Locus At1g23760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8403911 of line SALK_086372 Chr1:8403911
   
Insertion allele amplicon R204 - AW55
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer AW55: 5'-TTGCATCACTTCTCCTTTACTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8404544
   
Insertion allele amplicon LBb3 - AW56
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer AW56: 5'-GACATTAAGGATAAAATGCCGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8403545
   
wt amplicon (over InsPos) AW56 - AW55
wt amplicon start Chr1:8403545
wt amplicon end Chr1:8404544
wt amplicon size 1000 bp
Locus specific primer AW55: 5'-TTGCATCACTTCTCCTTTACTTCA-3'
2nd locus specific primer AW56: 5'-GACATTAAGGATAAAATGCCGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11