Genotyping and primer details


Genotyping and primer details for SALK_086656c - At5g67340
Line ID SALK_086656c
Locus At5g67340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26865680 of line SALK_086656c Chr5:26865680
   
Insertion allele amplicon LBb3 - 25ht
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 25ht: 5'-TCTTTTTCGTTTTCTTTGTTCGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26864747
   
wt amplicon (over InsPos) 11ix - 25ht
wt amplicon start Chr5:26864747
wt amplicon end Chr5:26865753
wt amplicon size 1007 bp
Locus specific primer 25ht: 5'-TCTTTTTCGTTTTCTTTGTTCGAT-3'
2nd locus specific primer 11ix: 5'-GAACCTCTGAAGTAGGTCCAACAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11