Genotyping and primer details


Genotyping and primer details for SALK_086996c - At3g05190
Line ID SALK_086996c
Locus At3g05190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1473825 of line SALK_086996c Chr3:1473825
   
Insertion allele amplicon LBb3 - 34fr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 34fr: 5'-AGGGTTACTTACAATGTTGGTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1474261
   
wt amplicon (over InsPos) 05hi - 34fr
wt amplicon start Chr3:1473501
wt amplicon end Chr3:1474261
wt amplicon size 761 bp
Locus specific primer 34fr: 5'-AGGGTTACTTACAATGTTGGTTGC-3'
2nd locus specific primer 05hi: 5'-ACATGCAGGTAAAAGAAGCAATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11