Genotyping and primer details


Genotyping and primer details for SALK_087083c - At2g15880
Line ID SALK_087083c
Locus At2g15880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:6919275 of line SALK_087083c Chr2:6919275
   
Insertion allele amplicon LBb3 - 13in
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 13in: 5'-AGAATATACTGGTGGTGGTGGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18318214
   
Insertion allele amplicon R212 - 40wt
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer 40wt: 5'-GAAAATGGTGAACTTCCTGATGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:6920114
   
wt amplicon (over InsPos) 01ho - 13in
wt amplicon start Chr2:6919323
wt amplicon end Chr1:18318214
wt amplicon size 606 bp
Locus specific primer 13in: 5'-AGAATATACTGGTGGTGGTGGTG-3'
2nd locus specific primer 01ho: 5'-GGAAGAGTTTGATATCTCTGGGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11