Genotyping and primer details


Genotyping and primer details for SALK_087356 - At4g17490
Line ID SALK_087356
Locus At4g17490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9753047 of line SALK_087356 Chr4:9753047
   
Insertion allele amplicon LBb3 - 80xo
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 80xo: 5'-TAAATGTTTTTGGGCAGCTATACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9754562
   
Insertion allele amplicon R204 - CE31
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CE31: 5'-GATGTTACGGTAATAAATCCGAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9752657
   
wt amplicon (over InsPos) CE32 - CE31
wt amplicon start Chr4:9752657
wt amplicon end Chr4:9753280
wt amplicon size 624 bp
Locus specific primer CE31: 5'-GATGTTACGGTAATAAATCCGAGC-3'
2nd locus specific primer CE32: 5'-CGTTTAGACTACGAGGATCAAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11