Genotyping and primer details


Genotyping and primer details for SALK_087410c - At4g26620
Line ID SALK_087410c
Locus At4g26620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13427928 of line SALK_087410c Chr4:13427928
   
Insertion allele amplicon R204 - CE41
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CE41: 5'-AGAAGCAGAAGCAGAGTTAACAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13427440
   
Insertion allele amplicon LBb3 - CE42
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CE42: 5'-GTACCTATTCTTTTGGAGCAGCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13428230
   
wt amplicon (over InsPos) CE42 - CE41
wt amplicon start Chr4:13427440
wt amplicon end Chr4:13428230
wt amplicon size 791 bp
Locus specific primer CE41: 5'-AGAAGCAGAAGCAGAGTTAACAGG-3'
2nd locus specific primer CE42: 5'-GTACCTATTCTTTTGGAGCAGCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11