Genotyping and primer details


Genotyping and primer details for SALK_087610 - At5g15600
Line ID SALK_087610
Locus At5g15600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5078243 of line SALK_087610 Chr5:5078243
   
Insertion allele amplicon LBb3 - 11ei
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 11ei: 5'-ATTCAAAACAATCCTCAAAACCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5078975
   
wt amplicon (over InsPos) 25gc - 11ei
wt amplicon start Chr5:5078177
wt amplicon end Chr5:5078975
wt amplicon size 799 bp
Locus specific primer 11ei: 5'-ATTCAAAACAATCCTCAAAACCAG-3'
2nd locus specific primer 25gc: 5'-TGTGTGTATCTCCTTGTTCCTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11