Genotyping and primer details


Genotyping and primer details for SALK_087857c - At4g13840
Line ID SALK_087857c
Locus At4g13840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8015496 of line SALK_087857c Chr4:8015496
   
Insertion allele amplicon LBb3 - 22jr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 22jr: 5'-CATACATTTACTCAGCCGAGACAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8016175
   
wt amplicon (over InsPos) 15ll - 22jr
wt amplicon start Chr4:8015130
wt amplicon end Chr4:8016175
wt amplicon size 1046 bp
Locus specific primer 22jr: 5'-CATACATTTACTCAGCCGAGACAG-3'
2nd locus specific primer 15ll: 5'-CTGAAGCCGAAGATTAGAACATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11