Genotyping and primer details


Genotyping and primer details for SALK_088429c - At5g08020
Line ID SALK_088429c
Locus At5g08020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2573875 of line SALK_088429c Chr5:2573875
   
Insertion allele amplicon LBb3 - 50kk
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 50kk: 5'-TGAAGAAATGTTTACCCCTGAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2573411
   
wt amplicon (over InsPos) 05mu - 50kk
wt amplicon start Chr5:2573411
wt amplicon end Chr5:2574343
wt amplicon size 933 bp
Locus specific primer 50kk: 5'-TGAAGAAATGTTTACCCCTGAAAC-3'
2nd locus specific primer 05mu: 5'-CTTTTTCTGACAACTTTCACACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11