Genotyping and primer details


Genotyping and primer details for SALK_090247c - At1g55740
Line ID SALK_090247c
Locus At1g55740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20835442 of line SALK_090247c Chr1:20835442
   
Insertion allele amplicon LBb3 - 39db
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 39db: 5'-ATAAAGTAGCAGCCTTTGAATGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20836022
   
wt amplicon (over InsPos) 22dz - 39db
wt amplicon start Chr1:20835269
wt amplicon end Chr1:20836022
wt amplicon size 754 bp
Locus specific primer 39db: 5'-ATAAAGTAGCAGCCTTTGAATGGA-3'
2nd locus specific primer 22dz: 5'-TGGACAACTAAATTTGCACCATAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11