Genotyping and primer details


Genotyping and primer details for SALK_090646c - At5g14120
Line ID SALK_090646c
Locus At5g14120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4556598 of line SALK_090646c Chr5:4556598
   
Insertion allele amplicon LBb3 - 16gq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16gq: 5'-ACAGTGACGAAATTCACAACAGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4555843
   
wt amplicon (over InsPos) 02ji - 16gq
wt amplicon start Chr5:4555843
wt amplicon end Chr5:4556654
wt amplicon size 812 bp
Locus specific primer 16gq: 5'-ACAGTGACGAAATTCACAACAGTC-3'
2nd locus specific primer 02ji: 5'-ATACTCACAGCCCATAGAGGAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11