Genotyping and primer details


Genotyping and primer details for SALK_093125 - At5g17540
Line ID SALK_093125
Locus At5g17540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5782667 of line SALK_093125 Chr5:5782667
   
Insertion allele amplicon LBb3 - 53fm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 53fm: 5'-GTACAACGATCCCTATCTCACCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5782186
   
wt amplicon (over InsPos) 15go - 53fm
wt amplicon start Chr5:5782186
wt amplicon end Chr5:5783047
wt amplicon size 862 bp
Locus specific primer 53fm: 5'-GTACAACGATCCCTATCTCACCAT-3'
2nd locus specific primer 15go: 5'-TAGTAACAATAATTTTGGGGACGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11