Genotyping and primer details


Genotyping and primer details for SALK_093557c - At4g14100
Line ID SALK_093557c
Locus At4g14100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8120917 of line SALK_093557c Chr4:8120917
   
Insertion allele amplicon LBb3 - 03ii
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 03ii: 5'-ACTAAATCTGCAGAGAGCTCCATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8121399
   
wt amplicon (over InsPos) 16ir - 03ii
wt amplicon start Chr4:8120495
wt amplicon end Chr4:8121399
wt amplicon size 905 bp
Locus specific primer 03ii: 5'-ACTAAATCTGCAGAGAGCTCCATT-3'
2nd locus specific primer 16ir: 5'-TTTTAAAAGGATTGTTGGTTGGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11