Genotyping and primer details


Genotyping and primer details for SALK_096152 - At4g17310
Line ID SALK_096152
Locus At4g17310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9686921 of line SALK_096152 Chr4:9686921
   
Insertion allele amplicon LBb3 - 05vw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 05vw: 5'-CCCAAAAGTGAAGCTTTCTAATCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9687403
   
wt amplicon (over InsPos) 63wi - 05vw
wt amplicon start Chr4:9686142
wt amplicon end Chr4:9687403
wt amplicon size 1262 bp
Locus specific primer 05vw: 5'-CCCAAAAGTGAAGCTTTCTAATCC-3'
2nd locus specific primer 63wi: 5'-AATCTTACAGCACTGAGCCACACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11