Genotyping and primer details


Genotyping and primer details for SALK_096484c - At3g44910
Line ID SALK_096484c
Locus At3g44910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:16392610 of line SALK_096484c Chr3:16392610
   
Insertion allele amplicon LBb3 - 78hw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 78hw: 5'-TTCTCTACGAATCAACATACGACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:16393237
   
wt amplicon (over InsPos) 01jm - 78hw
wt amplicon start Chr3:16392115
wt amplicon end Chr3:16393237
wt amplicon size 1123 bp
Locus specific primer 78hw: 5'-TTCTCTACGAATCAACATACGACG-3'
2nd locus specific primer 01jm: 5'-TTCTCTAGACGCTAGCAAATCTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11