Genotyping and primer details


Genotyping and primer details for SALK_097816 - At5g27850
Line ID SALK_097816
Locus At5g27850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:9873856 of line SALK_097816 Chr5:9873856
   
Insertion allele amplicon LBb3 - 29lu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29lu: 5'-GCTCCTAAGTCCGATGATGTCTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:9873386
   
wt amplicon (over InsPos) 09ng - 29lu
wt amplicon start Chr5:9873386
wt amplicon end Chr5:9874301
wt amplicon size 916 bp
Locus specific primer 29lu: 5'-GCTCCTAAGTCCGATGATGTCTAC-3'
2nd locus specific primer 09ng: 5'-CTTCTTAGACCTTGAATCCACGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11