Genotyping and primer details


Genotyping and primer details for SALK_099663c - At4g02380
Line ID SALK_099663c
Locus At4g02380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1046515 of line SALK_099663c Chr4:1046515
   
Insertion allele amplicon LBb3 - 92by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 92by: 5'-GTCGAAACTCCAGAGGTACAAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1045643
   
wt amplicon (over InsPos) 06dd - 92by
wt amplicon start Chr4:1045643
wt amplicon end Chr4:1046758
wt amplicon size 1116 bp
Locus specific primer 92by: 5'-GTCGAAACTCCAGAGGTACAAAAC-3'
2nd locus specific primer 06dd: 5'-GTGAACTCTCCAATGCTATCTTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11