Genotyping and primer details


Genotyping and primer details for SALK_100709 - At1g19170
Line ID SALK_100709
Locus At1g19170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6617642 of line SALK_100709 Chr1:6617642
   
Insertion allele amplicon LBb3 - 54qv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 54qv: 5'-GTGGTGTTTGATTTGAAGGATTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6617029
   
wt amplicon (over InsPos) 12rz - 54qv
wt amplicon start Chr1:6617029
wt amplicon end Chr1:6617714
wt amplicon size 686 bp
Locus specific primer 54qv: 5'-GTGGTGTTTGATTTGAAGGATTTT-3'
2nd locus specific primer 12rz: 5'-AAACATGAACTCGAAAGAGGAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11