Genotyping and primer details


Genotyping and primer details for SALK_102395c - At5g55660
Line ID SALK_102395c
Locus At5g55660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22541619 of line SALK_102395c Chr5:22541619
   
Insertion allele amplicon LBb3 - 46ew
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 46ew: 5'-CTCTGTAAAGAACCCAAAGAAACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22541931
   
wt amplicon (over InsPos) 44gc - 46ew
wt amplicon start Chr5:22541130
wt amplicon end Chr5:22541931
wt amplicon size 802 bp
Locus specific primer 46ew: 5'-CTCTGTAAAGAACCCAAAGAAACG-3'
2nd locus specific primer 44gc: 5'-AAGTTCTTAGGACTGCATTGTGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11