Genotyping and primer details


Genotyping and primer details for SALK_102810c - At3g47833
Line ID SALK_102810c
Locus At3g47833 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17650080 of line SALK_102810c Chr3:17650080
   
Insertion allele amplicon LBb3 - 15ck
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 15ck: 5'-ATGCATCTTTGAGGAGATTCAAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17649206
   
wt amplicon (over InsPos) 07dq - 15ck
wt amplicon start Chr3:17649206
wt amplicon end Chr3:17650175
wt amplicon size 970 bp
Locus specific primer 15ck: 5'-ATGCATCTTTGAGGAGATTCAAGT-3'
2nd locus specific primer 07dq: 5'-CCGGAGGAATAAGAATTGTATGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11