Genotyping and primer details


Genotyping and primer details for SALK_103154c - At1g76760
Line ID SALK_103154c
Locus At1g76760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28812721 of line SALK_103154c Chr1:28812721
   
Insertion allele amplicon LBb3 - 28ht
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 28ht: 5'-TCATATGATTGAGCCTGAAGATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28813259
   
wt amplicon (over InsPos) 10ix - 28ht
wt amplicon start Chr1:28812124
wt amplicon end Chr1:28813259
wt amplicon size 1136 bp
Locus specific primer 28ht: 5'-TCATATGATTGAGCCTGAAGATTG-3'
2nd locus specific primer 10ix: 5'-TCAATCTTCACCACCTGTATCTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11