Genotyping and primer details


Genotyping and primer details for SALK_103601c - At3g50720
Line ID SALK_103601c
Locus At3g50720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18847658 of line SALK_103601c Chr3:18847658
   
Insertion allele amplicon R204 - 56aq
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer 56aq: 5'-CTTGCAAGAGAAAAGACTCTTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18848222
   
wt amplicon (over InsPos) 03cb - 56aq
wt amplicon start Chr3:18847122
wt amplicon end Chr3:18848222
wt amplicon size 1101 bp
Locus specific primer 56aq: 5'-CTTGCAAGAGAAAAGACTCTTGGT-3'
2nd locus specific primer 03cb: 5'-TTCTTTGAATCGAAAAATCCTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11