Genotyping and primer details


Genotyping and primer details for SALK_104777c - At4g22680
Line ID SALK_104777c
Locus At4g22680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11923491 of line SALK_104777c Chr4:11923491
   
Insertion allele amplicon LBb3 - 16ck
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16ck: 5'-TAACTATCTCCGGCCTGACTTAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11923907
   
wt amplicon (over InsPos) 08dq - 16ck
wt amplicon start Chr4:11922953
wt amplicon end Chr4:11923907
wt amplicon size 955 bp
Locus specific primer 16ck: 5'-TAACTATCTCCGGCCTGACTTAAA-3'
2nd locus specific primer 08dq: 5'-CCTCATCTTTAGTAGTTTCGTCGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11