Genotyping and primer details


Genotyping and primer details for SALK_108611c - At3g63000
Line ID SALK_108611c
Locus At3g63000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:23284324 of line SALK_108611c Chr3:23284324
   
Insertion allele amplicon LBb3 - 20gq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 20gq: 5'-AATGGTTCCATAAGCTAAAAGGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:23284858
   
wt amplicon (over InsPos) 10mu - 20gq
wt amplicon start Chr3:23283958
wt amplicon end Chr3:23284858
wt amplicon size 901 bp
Locus specific primer 20gq: 5'-AATGGTTCCATAAGCTAAAAGGGT-3'
2nd locus specific primer 10mu: 5'-CCAAACCTTATCCACCAATAGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11