Genotyping and primer details


Genotyping and primer details for SALK_109250c - At4g19006
Line ID SALK_109250c
Locus At4g19006 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10410108 of line SALK_109250c Chr4:10410108
   
Insertion allele amplicon LBb3 - 45um
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 45um: 5'-ATGGAAAAAGCCTGCTTGATAGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10410623
   
wt amplicon (over InsPos) 60vr - 45um
wt amplicon start Chr4:10409582
wt amplicon end Chr4:10410623
wt amplicon size 1042 bp
Locus specific primer 45um: 5'-ATGGAAAAAGCCTGCTTGATAGTA-3'
2nd locus specific primer 60vr: 5'-TCACACATTCAAGAAGATTTCGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11