Genotyping and primer details


Genotyping and primer details for SALK_110774c - At4g36210
Line ID SALK_110774c
Locus At4g36210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17133037 of line SALK_110774c Chr4:17133037
   
Insertion allele amplicon LBb3 - 07nj
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 07nj: 5'-CAATCTTCTATCTTGCACATTGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17133528
   
wt amplicon (over InsPos) 12or - 07nj
wt amplicon start Chr4:17132424
wt amplicon end Chr4:17133528
wt amplicon size 1105 bp
Locus specific primer 07nj: 5'-CAATCTTCTATCTTGCACATTGGA-3'
2nd locus specific primer 12or: 5'-GTATTTCCTCTCCCTCCTTCATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11