Genotyping and primer details


Genotyping and primer details for SALK_116215 - At1g31300
Line ID SALK_116215
Locus At1g31300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11195377 of line SALK_116215 Chr1:11195377
   
Insertion allele amplicon R204 - CG03
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CG03: 5'-TTCAAAGAATCGAATGGAATAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11194604
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11