Genotyping and primer details


Genotyping and primer details for SALK_116695c - At1g77440
Line ID SALK_116695c
Locus At1g77440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29097857 of line SALK_116695c Chr1:29097857
   
Insertion allele amplicon LBb3 - 44dr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 44dr: 5'-GATAACAAACCCTTCATTTGCACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29097005
   
wt amplicon (over InsPos) 26lt - 44dr
wt amplicon start Chr1:29097005
wt amplicon end Chr1:29098225
wt amplicon size 1221 bp
Locus specific primer 44dr: 5'-GATAACAAACCCTTCATTTGCACT-3'
2nd locus specific primer 26lt: 5'-AGAACGAACCACAATATCCAGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11