Genotyping and primer details


Genotyping and primer details for SALK_121247 - At1g23560
Line ID SALK_121247
Locus At1g23560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8353990 of line SALK_121247 Chr1:8353990
   
Insertion allele amplicon LBb3 - 05du
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 05du: 5'-GCTTATATTTTGACTGGCCTCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8353230
   
wt amplicon (over InsPos) 15fg - 05du
wt amplicon start Chr1:8353230
wt amplicon end Chr1:8354335
wt amplicon size 1106 bp
Locus specific primer 05du: 5'-GCTTATATTTTGACTGGCCTCAAG-3'
2nd locus specific primer 15fg: 5'-AGGTGCGTAAGGTACACTCAATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11