Genotyping and primer details


Genotyping and primer details for SALK_122307 - At4g37880
Line ID SALK_122307
Locus At4g37880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17810991 of line SALK_122307 Chr4:17810991
   
Insertion allele amplicon LBb3 - 29ew
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29ew: 5'-TGGTATGATTTTTGCTTGTCTCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17810124
   
wt amplicon (over InsPos) 27dr - 29ew
wt amplicon start Chr4:17810124
wt amplicon end Chr4:17811156
wt amplicon size 1033 bp
Locus specific primer 29ew: 5'-TGGTATGATTTTTGCTTGTCTCAG-3'
2nd locus specific primer 27dr: 5'-TGAAACTGAAACTCCTCGGATAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11