Genotyping and primer details


Genotyping and primer details for SALK_124412c - At5g41730
Line ID SALK_124412c
Locus At5g41730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16686646 of line SALK_124412c Chr5:16686646
   
Insertion allele amplicon LBb3 - 37mv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 37mv: 5'-TGTTCTTTGGTCTCATTTTGATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16687267
   
wt amplicon (over InsPos) 21oj - 37mv
wt amplicon start Chr5:16686357
wt amplicon end Chr5:16687267
wt amplicon size 911 bp
Locus specific primer 37mv: 5'-TGTTCTTTGGTCTCATTTTGATTG-3'
2nd locus specific primer 21oj: 5'-TGCAAAACTATCACCTAACCATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11