Genotyping and primer details


Genotyping and primer details for SALK_125666c - At4g14723
Line ID SALK_125666c
Locus At4g14723 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8440716 of line SALK_125666c Chr4:8440716
   
Insertion allele amplicon LBb3 - 33uf
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 33uf: 5'-ACCACAACACTTGTCTCTCTGCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8440114
   
wt amplicon (over InsPos) 58vr - 33uf
wt amplicon start Chr4:8440114
wt amplicon end Chr4:8441376
wt amplicon size 1263 bp
Locus specific primer 33uf: 5'-ACCACAACACTTGTCTCTCTGCTA-3'
2nd locus specific primer 58vr: 5'-CGTAGTTGTGGTAGTTTACCATTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11