Genotyping and primer details


Genotyping and primer details for SALK_126880c - At1g67070
Line ID SALK_126880c
Locus At1g67070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25042190 of line SALK_126880c Chr1:25042190
   
Insertion allele amplicon LBb3 - 16ht
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16ht: 5'-GACTCGTTGTCCTGTATTAGCCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25042488
   
wt amplicon (over InsPos) 01jt - 16ht
wt amplicon start Chr1:25041898
wt amplicon end Chr1:25042488
wt amplicon size 591 bp
Locus specific primer 16ht: 5'-GACTCGTTGTCCTGTATTAGCCTC-3'
2nd locus specific primer 01jt: 5'-TCAGATTTCTCCTCCACGTCTACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11